Scalable fabrication of Immunosensors based on Carbon Nanotube Polymer Composites
E. Mendoza, J. Orozco, C. Jiménez-Jorquera, A. B. González-Guerrero, A. Calle, L. M. Lechuga and C. Fernández-Sánchez
Nanotechnology, 19, 2008, 075102/1-6 - DOI: 10.1088/0957-4484/19/7/075102
Abstract: In this work we present the fabrication and characterization of immunosensors based on polystyrene (PS)–multiwalled carbon nanotube (MWCNT) composites. The electrochemical properties of the sensors have been investigated and show that the surface area is increased upon addition of the MWCNT–PS layer. Furthermore, a plasma activation process is used to partially remove the PS and expose the MWCNTs. This results in a huge increase in the electrochemical area and opens up the possibility of binding biomolecules to the MWCNT wall. The MWCNTs have been functionalized covalently with a model antibody (rabbit IgG). The biosensors have been tested using amperometric techniques and show detection limits comparable to standard techniques such as ELISA.