Molecular inversion probe-based SPR biosensing for specific, label-free and real-time detection of regional DNA methylation
L. G. Carrascosa, A. Ali Ibn Sina, R. Palanisamy, B. Sepulveda, M. A. Otte, S. Rauf, M. J. A. Shiddikya and M. Trau
Chemical Communications, 50, 2014, 3585-3588 - DOI: 10.1039/C3CC49607D
Abstract: DNA methylation has the potential to be a clinically important biomarker in cancer. This communication reports a real-time and label-free biosensing strategy for DNA methylation detection in the cancer cell line. This has been achieved by using surface plasmon resonance biosensing combined with the highly specific molecular inversion probe based amplification method, which requires only 50 ng of bisulfite treated genomic DNA.