Low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine
D. Martens, P. Ramirez-Priego , M. S. Murib, A.A. Elamin, A.B. Gonzalez-Guerrero, M. Stehr, F. Jonas, B. Anton, N. Hlawatsch, P. Soetaert, R. Vos, A. Stassen, S. Severi, W. Van Roy, R. Bockstaele, H. Becker, M. Singh, L.M. Lechuga, P. Bienstman
Analytical Methods, 2018 - DOI: 10.1039/C8AY00666K
Abstract: We present a low-cost integrated nanophotonic lab-on-a-chip platform suitable for point-of-care (POC) biomarker analysis. The sensor chip included in the platform contains multiplexed Mach-Zehnder interferometers with an on-chip optical spectral analyser consisting of an arrayed-waveguide grating. The sensor chip is fabricated in silicon nitride material, which renders it compatible with consumer-electronics-grade sources and detectors, leading to the possibility of low-cost instrumentation. The nanophotonic sensor chip exhibits a detection limit of 6.10-6 RIU (Refractive Index Units), which is in the same order of magnitude as the reported values for the evanescent wave sensors at the state-of-the-art. The sensor chip is biofunctionalised with specific bioreceptors and integrated in a polymer microfluidics cartridge. The POC instrumentation platform contains the optical excitation and read-out sub-systems and a dedicated on-board software for a real-time analysis of patient samples. To demonstrate the versatility of the platform, we present results both on the detection of an antigen related to tuberculosis directly in urine samples using a laboratory prototype and on the detection of a protein biomarker (CRP) related to inflammation using the integrated instrument.