Label-free bacteria quantification in blood plasma by a bioprinted microarray based interferometric point-of-care device

P. Dey, N. Fabri-Faja, O. Calvo-Lozano, R. Terborg, A. Belushkin, F. Yesilköy, A. Fàbrega, J. C. Ruiz-Rodriguez, R. Ferrer, J. J. González-López, M. C. Estévez, H. Altug, V. Pruneri, L. M. Lechuga

ACS Sensors, 4, 2019, 52-60 - DOI: 10.1021/acssensors.8b00789

http://pubs.acs.org/doi/10.1021/acssensors.8b00789

Abstract: Existing clinical methods for bacteria detection lack in speed, sensitivity and importantly in Point-of-
Care (PoC) applicability. Thus, finding ways to push the sensitivity of clinical PoC biosensing
technologies is crucial. Aiming that, we here report a portable PoC device based on Lens-free
Interferometric Microscopy (LIM). The device employs high performance nanoplasmonics and
custom bioprinted microarrays and is capable of direct label-free bacteria (E. coli) quantification. With
only one-step sample handling we offer a sampletodata turnaround time of 40 minutes. Our
technology features detection sensitivity of a single bacterial cell both in buffer and diluted blood
plasma and is intrinsically limited by the number of cells present in the detection volume. When
employed in a hospital setting, the device has enabled accurate categorization of sepsis patients
(infectious SIRS) from control groups (healthy individuals and non-infectious SIRS patients) without
false positives/negatives. User-friendly on-site bacterial clinical diagnosis can thus become a reality

Label-free bacteria quantification in blood plasma by a bioprinted microarray based interferometric point-of-care device
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