Direct and label-free detection of the human growth hormone in urine by an ultrasensitive bimodal waveguide biosensor
A. B. González-Guerrero, J. Maldonado, S. Dante, D. Grajales, and L. M. Lechuga
J. of Biophotonics,, 10 (1), 2017, 61-67 - DOI: 10.1002/jbio.201600154
Abstract: A label-free interferometric transducer showing a theoretical detection limit for homogeneous sensing of 5 × 10–8 RIU, being equivalent to a protein mass coverage resolution of 2.8 fg mm–2, is used to develop a high sensitive bio- sensor for protein detection. The extreme sensitivity of this transducer combined with a selective bioreceptor layer enables the direct evaluation of the human growth hormone (hGH) in undiluted urine matrix in the 10 pg mL–1 range.